Bacterial Secretion Systems: Methods and Protocols

This  second edition details new and updated protocols that cover techniques  used to study secretion systems. Chapters focus on identifying and  localizing the different subunits, defining interactions within  subunits, monitoring conformational changes, purifying and imaging of  large complexes, defining the assembly pathway by fluorescence  microscopy and the role of energy during assembly and/or secretion,  identifying secreted effectors as well as using reporters to follow  effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective  topics, lists of the necessary materials and reagents, step-by-step,  readily reproducible laboratory protocols, and key tips on  troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Secretion Systems: Methods and Protocols- Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.