Poly(ADP-Ribose) Polymerase: Methods and Protocols

This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in  the biology of eukaryotes and their relevance to human health. Beginning  with a section on the detection and quantification of poly(ADP-ribose)  polymer (pADPr), the book continues by delving into the identification  of protein targets, functional analysis, the poly(ADP-ribosyl)ating  pathway in chromatin and genes expression, as well as the use of animal  models and PARP1 inhibitor design and testing, and more. Written for the  highly successful Methods in Molecular Biology series, chapters include  introductions to their respective topics, lists of the necessary  materials and reagents, step-by-step and readily reproducible laboratory  protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative  and up-to-date, Poly(ADP-Ribose) Polymerase: Methods and Protocols,  Third Edition presents essential new and classical methods for studying  the pADPr-pathway.