Antisense RNA Design, Delivery, and Analysis

This  open access volume gathers a variety of models, delivery systems, and  approaches that can be used to assess RNA technology for exploiting  antisense as a therapeutic intervention. Beginning with a section on the  design of antisense technology and their delivery, the book continues  by covering model systems developed to evaluate efficacy, both in vivo  and in vitro, as well as methods to evaluate preclinically the toxicity  associated with these new potential drugs, and intellectual property  considerations. Written for the highly successful Methods in Molecular  Biology series, chapters include introductions to their respective  topics, lists of the necessary materials and reagents, step-by-step,  readily reproducible laboratory protocols, and tips on troubleshooting  and avoiding known pitfalls.

Authoritative and practical,  Antisense RNA Design, Delivery, and Analysis provides basic knowledge  and a large collection of methods to facilitate the work of newcomers to  this vibrant and expanding field.

This book was conceived  thanks to the network DARTER (Delivery of Antisense RNA Therapeutics).  DARTER is funded by the EU Cooperation of Science and Technology (COST),  which aims to enhance interaction and collaborations between  researchers in Europe and other countries.