Multi-Dimensional Liquid Chromatography: Principles, Practice, and Applications

Two-dimensional liquid chromatography (2D-LC) is finding increasingly  wide application principally due to the analysis of mixtures of moderate  to high complexity. Many industries are developing increasingly complex  products that are challenging the separation capabilities of  state-of-the-art 1D-LC and need new analytical methodologies with  substantially more resolving power, and 2D-LC meets that need.

This  text, organized by two leaders in the field, establishes a sound  fundamental basis for the principles of the technique, followed by a  discussion of important practical considerations. The book begins with  an introduction to multi-dimensional separations and a discussion of the  history and development of the technique over the past 40 years,  followed by several chapters that provide a theoretical basis for  development of 2D-LC methods, including foundational concepts regarding  separation complementarity, under-sampling, and dynamics of liquid  chromatography separations. Instrumentation for 2D-LC is discussed  extensively, including practical aspects such as interface selection and  setup. Building on this foundation, two separate chapters are focused  on method development for non-comprehensive and comprehensive  separations, followed by a chapter dedicated to data analysis. Finally,  applications of 2D-LC in several fields ranging from pharmaceutical  analysis to polymer science are summarized.

The book is an  important resource for both students and practitioners who are already  using 2D-LC or are interested in getting started in the field.

Key Features:
Demonstrates  the conditions under which a 2D-LC method should be considered as an  alternative to a 1D-LC method. Establishes a sound fundamental basis of  the principles of the technique, followed by guidelines for method  optimization. Provides a single source for technical knowledge advances  and practical guidance described in recent literature. Assists with the  initial decision to develop a 2D-LC method. Guides the reader in  developing a high-quality method that meets the needs of their  application.