Signal Transduction Immunohistochemistry: Methods and Protocols

This new edition collects diverse protocols compiled by experts from  different areas of research as well as by biotech researchers developing  novel technologies in the area of immunohistochemistry (IHC). After a  few chapters to help orient novices entering the biomedical arena, the  book includes methods chapters covering multiplex IHC including  fluorescence and chromogenic techniques, combination of IHC with In Situ  Hybridization (ISH), wavelet transform approach for organelle tracking,  transcription factors in human stem cells, differentiation of  mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the  human brain, tissue fixation, permeabilization, and cryo-preservation,  as well as other topics. Written for the highly successful Methods in  Molecular Biology series, chapters include introductions to their  respective topics, lists of the necessary materials and reagents,  step-by-step and readily reproducible laboratory protocols, and tips on  troubleshooting and avoiding known pitfalls.
Authoritative and  up-to-date, Signal Transduction Immunohistochemistry: Methods and  Protocols, Third Edition aims to arm novices and experts with vital  protocols they can use either as-is or tailor them for specific  experimental needs.